PlexPlus® Technology
Access this novel two-temperature in-channel multiplexing — Only with SpeeDx!
Patented PlexPlus® technology represents the cutting-edge of PCR multiplexing, with innovative in-channel multiplexing delivering twice the results from existing equipment. Using a completely novel approach with temperature-dependent probes, PlexPlus® delivers two independent results per fluorescent channel—effectively doubling the output of conventional qPCR systems. The first SpeeDx PlexPlus® assay enables laboratories to detect 14 targets in a single well, using either 96- or 384-well plates. Transform the economics of multiplexed testing in your lab while maintaining the accuracy and reliability that SpeeDx is known for worldwide.
Patented PlexPlus® technology represents the cutting-edge of PCR multiplexing, with innovative in-channel multiplexing delivering twice the results from existing equipment. Using a completely novel approach with temperature-dependent probes, PlexPlus® delivers two independent results per fluorescent channel—effectively doubling the output of conventional qPCR systems. The first SpeeDx PlexPlus® assay enables laboratories to detect 14 targets in a single well, using either 96- or 384-well plates. Transform the economics of multiplexed testing in your lab while maintaining the accuracy and reliability that SpeeDx is known for worldwide.
PlexPlus® Technology — Doubling the Power of PCR
High multiplexing in a single reaction eliminates the need for multiple wells, reducing reagent consumption and hands-on time, and increases sample throughput.
Amplification Streamlined
Achieve comparable sensitivity and specificity while detecting twice as many targets per channel.
Outstanding Accuracy
PlexZyme® enzyme technology ensures consistent performance across millions of tests, while adhering to tight manufacturing specifications.
Manufacturing Precision
Deploy on your existing qPCR platforms with standard run protocols—no specialized equipment, training, or workflow changes required.
Unmatched Simplicity
How it works
PlexPlus® utilizes pairs of probes that produce signals at different temperatures during the PCR reaction. Within each fluorescent channel, two probes can be used to create fluorescence when they bind — one probe specifically binds and produces a signal at a lower temperature indicating the presence of the first target, while a second probe is designed to produce a signal at a higher temperature, thus indicating the presence of a second target.
This elegant solution bypasses the traditional limitation of needing a separate fluorescent channel for each target. The result: laboratories can multiplex twice as many targets using their existing qPCR platforms without purchasing new equipment or changing workflows. Furthermore, even more than double the targets can be achieved if multiple targets[1] are reported together as a single readout.
Key Technical Features
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Temperature-dependent universal probe signalling
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Two target readouts per fluorescent channel
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Compatible with existing qPCR platforms
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No specialized equipment required
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User workflow no different than conventional PCR
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High sensitivity and specificity[1], like other SpeeDx assays
Transform Your
Laboratory Economics
Significantly Reduce Costs
Save by running comprehensive in-channel multi-target panels instead of traditional single-target tests.
Double Platform Capacity
Eliminate the need for second well reactions and process twice as many samples per run by detecting two targets per channel.
Faster Time-to-Result
Process >2,230 specimens in < 8 hours with PlexPlus® technology*
Maximize Equipment ROI
Unlock more value from existing qPCR platforms without further capital investment.
Scale With Confidence
Choose 96- or 384-well format. Proven in high-throughput laboratories processing thousands of samples daily.
Flexible Implementation
Available as off-the-shelf products, custom development, or a technology partnership. Combine with PlexPrime® and/or InSignia® to fulfil your most ambitious programs.
Partner with SpeeDx — the only place you can access cutting-edge PlexPlus® technology!
Contact the Technology team?
SpeeDx offers the technology, expertise, and partnership models to accelerate your diagnostic success.
Footnote
* Based on theoretical calculations of instrumentation throughput, run time and require hands-on time.
References:
1. Hasick et. al. PLoS One. 2022 Mar 9;17(3):e0263329